The urine samples will either be for random specimen sampling or for 24-hr sampling. Random samples should be collected in a clean container unless a culture is indicated in which a sterile container is mandatory.
All random urines should be collected utilizing a “midstream urine” collection technique. This involves (1) cleansing and rinsing the urethral area; (2) starting to void for at least several ml into the toilet; and then (3) start collecting the urine into the cup without stopping the voiding. The specimen should be stored as indicated in “Test Catalog”.
To collect a 24-hr urine sample, the patient should be given a clean collection container capable of holding all urine passed for 24 hours. The patient should be instructed to void completely in the morning, making note of the time, and discard the urine unless the specimen is to be utilized for bacteriology exams. For 24 hours, all subsequent urine should be collected in one container and should be kept cool and protected from light.
The patient should collect the last of the urine sample at the next morning. The submitting laboratory should measure the volume of the 24-hr urine sample and note it on the request form, along with the patient’s weight and height. The specimen should be stored as indicated in the “Test Catalog” , the amount of urine indicated there should be forwarded to Delta Lab.
Collecting Specimens for Feces Sampling:
Feces (stool) collected for examination should contain no water, urine, or blood. Feces should be caught in a clean container and stored or mixed with appropriate preservative as indicated in the “Test Catalog”. Feces for stool culture analysis must be collected in a sterile container.
To collect 24-hr feces sample, feces should be collected for a 24-hour period in a clean container whose empty weight has been noted. No urine, blood, or water should enter the container. The full container and feces should be weighed. The net weight of the feces should be indicated on the request form. The appropriate quantity of feces should be removed from the 24-hr collection and stored as indicated in the “Test Catalog”.
Specimen Preparation for Metal Analysis:
When multiple blood samples are scheduled for collection from one patient, the trace metal specimens should be collected first. Once the phlebotomy needle has punctured another rubber stopper, it is contaminated & should not be used for trace metal specimen collection.
Use an alcohol swab to clean the venipuncture site. Avoid iodine-containing disinfectants. Use stainless steel phlebotomy needles.
Blood specimens for serum testing should be collected in the Monoject (royal blue top) trace element blood collection tube ( if unavailable in your clinic or hospital, Delta Lab can supply this item). Allow the specimen to clot for 30 min. Centrifuge the specimen to separate serum from the cellular fraction.
Remove the stopper and carefully pour the serum into 5 mL metal-free, screw-capped, polypropylene vial. Avoid transfer of the cellular components of blood. Do not insert a pipette into the serum to accomplish transfer, do not ream the sample with a wooden stick to assist with serum transfer. Place the cap on the polypropylene vial tightly, attach a specimen label, and send specimen to the laboratory refrigerated or frozen.
Specimen for whole-blood testing should be collected in the blood collection tube, containing EDTA as an anticoagulant. Leave the specimen in the tube. Attach an identification label and send the specimen to the laboratory at cool temperature. Alternatively, pediatric specimens can be drawn in a Becton-Dickinson Microtainer, with EDTA Anticoagulant. An absolute volume of 0.5 mL is required for testing for each analyte ordered.
Specimen Preparation for Cytogenetics:
– Chromosome Analysis for Congenital Disorders:
Use green top (lithium heparin) tube. Collect 7-10 mL (for pediatric 2.5 – 5mL in a sodium heparin tube).
Invert to mix. Refrigerate for shipping.
DO NOT FREEZE.
– Products of Conception or Stillbirth (Please contact Delta Lab before sending the sample).
Obtain 1 cc of muscle and fascia from thigh or 1 cc of placental tissue. Place in sterile screw-topped bottle with Hanks balanced salt solution. KEEP COOL. Delta Lab can supply the required container.
– Skin Biopsy (Please contact Delta Lab before sending the sample).
Wash skin with antiseptic such as pHisoHex. Wash well with sterile water. Obtain punch biopsy 3 mm x 5 mm. Place in tissue culture media. Keep refrigerated.
– Solid Tumors or Lymph Nodes (Please contact Delta Lab before sending the sample)
Obtain 0.5 cc piece of tissue and place in tissue culture medium. Refrigerate.
– Chromosome Breakage:
Obtain blood as in chromosome analysis for congenital Disorders (see above).
– Chromosome Analysis Consultation:
Offered on the basis of Fluorescent, in-Situ Hybridization (FISH) Techniques and other methods. For specimen collection, call Delta Lab for special instruction.
Specimen Preparation for Viral Culture:
– Blood Specimen:
Draw blood in lavender top (EDTA) tube(s) and send 5 mL (for pediatric 0.5 mL) of EDTA whole blood refrigerated. DO NOT FREEZE. Maintain sterility and forward promptly.
– Lesions an on skin specimens:
Send specimen refrigerated in a screw-capped, sterile container. DO NOT FREEZE. Maintain sterility and forward promptly.
– Sputum and Biopsy Specimen (Brain, Liver or Lung):
Send specimen refrigerated. DO NOT FREEZE. Maintain sterility and forward promptly
– Spinal Fluid and Urine Specimens (Please contact Delta Lab before sending the sample)
Send 2mL (pediatric 0.5mL) of spinal fluid or urine. Send specimen refrigerated. DO NOT FREEZE. Maintain sterility & forward promptly.
– Swab Specimens of Cervix, Rectum, Urethra, Vagina & other Genital Sites (Please contact Delta Lab before sending the sample)
Collect specimen using Culture swab. Break medium ampuole to moisten swab. Send specimen refrigerated. DO NOT FREEZE